5ZWL

Crystal structure of the gamma - epsilon complex of photosynthetic cyanobacterial F1-ATPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-27
Detector	RAYONIX MX300HE
Wavelength(s)	0.900
Spacegroup name	P 41 21 2
Unit cell lengths	71.556, 71.556, 204.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.004 - 1.980
R-factor	0.2069
Rwork	0.205
R-free	0.24200
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.244
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.004	2.010
High resolution limit [Å]	1.980	1.980
Rmerge	0.055	0.866
Number of reflections	37645	1666
<I/σ(I)>	38.1	1.1
Completeness [%]	98.5	89.6
Redundancy	6.8	5
CC(1/2)	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	sodium/potassium phosphate buffer (pH 6.0), sodium chloride, PEG 4000