5ZW7
FAD-PigA complex at 1.3 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-06 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.228, 85.624, 145.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.300 |
| R-factor | 0.1139 |
| Rwork | 0.114 |
| R-free | 0.12740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zw2 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.070 | 0.483 |
| Number of reflections | 130943 | 12927 |
| <I/σ(I)> | 42.8 | 3.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 1.7M (NH4)2SO4, 0.2M NaCl, 0.1M Na-cacodylate |
