5ZW3
Crystal Structure of TrmR from B. subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.874, 116.738, 43.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.270 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gpy |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.415 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.310 |
| High resolution limit [Å] | 2.270 | 6.160 | 2.270 |
| Rmerge | 0.091 | 0.060 | 0.413 |
| Rmeas | 0.100 | 0.067 | 0.462 |
| Rpim | 0.040 | 0.028 | 0.202 |
| Total number of observations | 113363 | ||
| Number of reflections | 18494 | 949 | 878 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.2 | 90.7 | 97.6 |
| Redundancy | 6.1 | 5.5 | 5.1 |
| CC(1/2) | 0.997 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.1 M Ammonium tartrate dibasic (pH 7.0) |
