5ZVX
Crystal structure of K86A mutant of phosphomannose isomerase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.190, 91.730, 116.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.580 - 1.700 |
| R-factor | 0.1663 |
| Rwork | 0.164 |
| R-free | 0.20569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h1m |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.918 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.580 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 43702 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.8 | 298 | 0.1 M Magnesium acetate tetrahydrate, 0.2 M Sodium cacodylate pH 6.8, 20% PEG 8000,5% dioxane |
