5ZVP
Aspergillus fumigatus Rho1 F25N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 253 |
| Detector technology | PIXEL |
| Collection date | 2016-03-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 43 |
| Unit cell lengths | 53.815, 53.815, 62.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.820 - 1.420 |
| R-factor | 0.15683 |
| Rwork | 0.155 |
| R-free | 0.18482 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.297 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.470 |
| High resolution limit [Å] | 1.400 | 1.420 |
| Number of reflections | 31770 | |
| <I/σ(I)> | 18.79 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 37.1% w/v PEG 5000 MME, 150 mM Tris pH 8.0, 40 mM magnesium sulphate. |
