Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 63 |
| Unit cell lengths | 135.391, 135.391, 45.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.700 - 2.700 |
| R-factor | 0.19837 |
| Rwork | 0.195 |
| R-free | 0.24975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y8t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.707 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 2.840 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.779 |
| Rpim | 0.041 | |
| Number of reflections | 12591 | |
| <I/σ(I)> | 17.2 | 2.8 |
| Completeness [%] | 93.8 | |
| Redundancy | 9.3 | 9.2 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Ammonium sulfate, PEG2000, Ethanol |
