5ZV3

Crystal structure of human anti-tau antibody CBTAU-28.1 in complex with its tau peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	77.241, 49.423, 61.375
Unit cell angles	90.00, 105.55, 90.00

Refinement procedure

Resolution	41.223 - 2.093
R-factor	0.1906
Rwork	0.187
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3QOT
RMSD bond length	0.007
RMSD bond angle	0.952
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.090	2.100
Rmerge	0.120	0.520
Rmeas	0.150
Rpim	0.080	0.650
Number of reflections	25910	3146
<I/σ(I)>	12.7	1.1
Completeness [%]	97.5	78.4
Redundancy	3.4	2.4
CC(1/2)	0.985	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.085M Tris-HCl, pH 8.5, 0.17M sodium acetate, 25.5% PEG 4000