5ZUT
Crystal Structure of Yeast PCNA in Complex with N24 Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.350, 84.030, 58.150 |
| Unit cell angles | 90.00, 93.62, 90.00 |
Refinement procedure
| Resolution | 58.030 - 2.820 |
| R-factor | 0.3301 |
| Rwork | 0.326 |
| R-free | 0.39660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6B8I 5da7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.063 |
| Data reduction software | XDS |
| Data scaling software | Aimless (3.24) |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.030 | 58.030 | 2.900 |
| High resolution limit [Å] | 2.830 | 12.630 | 2.830 |
| Rmerge | 0.133 | 0.109 | 0.512 |
| Number of reflections | 9212 | ||
| Completeness [%] | 98.7 | 98.6 | 99.4 |
| Redundancy | 6.9 | 6.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | n-Octyl-b-D-glu, NaCi pH 5.5, PEG 3350 |
