5ZUR
Achromobacter Dh1f Bacterioferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 158.665, 158.665, 118.061 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.700 - 1.950 |
| R-factor | 0.14437 |
| Rwork | 0.142 |
| R-free | 0.18707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fl0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.689 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.123 | 0.551 |
| Number of reflections | 54638 | 5355 |
| <I/σ(I)> | 14.74 | 2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 8.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2 M Sodium acetate tetrahydrate, 0.1 M sodium cacodylate tetrahydrate pH 6.5, 30%(w/v), PEG 8000 |
