5ZUQ
P domain of GII.17-1978
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.590, 101.635, 82.595 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.070 - 1.990 |
| R-factor | 0.19381 |
| Rwork | 0.191 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pun |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.070 | 2.030 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.128 | 0.512 |
| Number of reflections | 20206 | 1027 |
| <I/σ(I)> | 23.1 | 3.4 |
| Completeness [%] | 94.7 | 96.9 |
| Redundancy | 5.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% (w/v) polyethylene glycol (PEG) 3350, 0.2M MgCl2 |
