5ZU6
A CBM32 derived from alginate lyase B (AlyB-OU02)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-11-25 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.849, 60.679, 66.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.940 - 1.400 |
| R-factor | 0.19023 |
| Rwork | 0.189 |
| R-free | 0.21161 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zu5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.876 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.120 | 0.470 |
| Number of reflections | 35712 | 3517 |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 1.6M sodium citrate tribasic dihydrate pH 6.5 |
