5ZTP
Carbonic anhydrase from Glaciozyma antarctica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-26 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.058, 82.058, 99.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.950 - 2.960 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3las |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.739 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.010 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Number of reflections | 8380 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 20 mM tris hcl, 50 mM NaCl, 10% glycerol, 0.05 mM Ammonium sulfate |
