5ZT4
Crystal structure of H99A mutant of phosphomannose isomerase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.220, 91.660, 116.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.350 - 1.700 |
| R-factor | 0.18183 |
| Rwork | 0.180 |
| R-free | 0.21552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h1m |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.848 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.350 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 43599 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.8 | 298 | 0.1 M Magnesium acetate tetrahydrate, 0.2 M Sodium cacodylate pH 6.8, 20% PEG 8000,5% dioxane |
