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5ZSM

Crystal structure of monkey TLR7 in complex with GGUCCC

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL41XU
Synchrotron siteSPring-8
BeamlineBL41XU
Temperature [K]100
Detector technologyPIXEL
Collection date2017-07-16
DetectorDECTRIS PILATUS3 6M
Wavelength(s)1.0000
Spacegroup nameP 21 21 21
Unit cell lengths99.655, 139.173, 150.794
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution47.360 - 2.500
R-factor0.23055
Rwork0.228
R-free0.27346
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.011
RMSD bond angle1.577
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0230)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.550
High resolution limit [Å]2.5002.500
Number of reflections69491
<I/σ(I)>12.6
Completeness [%]99.9
Redundancy6.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP293PEG 8000, ammonium sulfate, sodium citrate pH 5.0, Tris-HCl pH 7.5 and NaCl

219869

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