5ZS3
Small heat shock protein from M. marinum:Form-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 91.140, 91.140, 91.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.446 - 2.001 |
| R-factor | 0.1984 |
| Rwork | 0.196 |
| R-free | 0.24530 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.226 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.450 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.121 | |
| Rpim | 0.052 | 0.531 |
| Number of reflections | 8114 | 634 |
| <I/σ(I)> | 14.1 | 3.4 |
| Completeness [%] | 93.4 | 100 |
| Redundancy | 12.3 | 11 |
| CC(1/2) | 0.997 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 2.1 M DL-Malic acid pH 7.0 |
