5ZS0
Structure of glycoprotein B Domain IV of pseudorabies virus with 7B11 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 128.907, 128.907, 194.914 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.290 |
| R-factor | 0.2439 |
| Rwork | 0.241 |
| R-free | 0.30970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ys2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.695 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.120 | 2.696 |
| Number of reflections | 14967 | |
| <I/σ(I)> | 21.35 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ammonium phosphate dibasic and 20% w/v Polyethylene glycol 3,350 |
