5ZRZ

Crystal Structure of EphA5/SAMD5 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97961
Spacegroup name	C 1 2 1
Unit cell lengths	98.195, 29.651, 54.170
Unit cell angles	90.00, 109.95, 90.00

Refinement procedure

Resolution	50.000 - 1.890
R-factor	0.1957
Rwork	0.194
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kka
RMSD bond length	0.015
RMSD bond angle	1.673
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.890	5.160	1.900
Rmerge	0.053	0.026	0.771
Rmeas	0.064	0.032	0.931
Rpim	0.035	0.018	0.516
Number of reflections	11829	559	575
<I/σ(I)>	12.3
Completeness [%]	99.1	87.1	100
Redundancy	3.2	3	3.2
CC(1/2)		0.998	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	289	0.2M ammonium acetate, 0.1M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3350