5ZRY

Crystal Structure of EphA6/Odin Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	38.985, 85.042, 98.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.300
R-factor	0.1703
Rwork	0.170
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kka 2lmr
RMSD bond length	0.010
RMSD bond angle	1.523
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.320
High resolution limit [Å]	1.300	3.530	1.300
Rmerge	0.054	0.035	0.903
Rmeas	0.059	0.039	0.987
Rpim	0.024	0.017	0.390
Number of reflections	79398	3802	3792
<I/σ(I)>	11.5
Completeness [%]	97.8	87	95.1
Redundancy	5.9	5.4	5.7
CC(1/2)		0.997	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	0.1M HEPES sodium pH 7.5, 1.5M lithium sulfate monohydrate