5ZRP
M. smegmatis antimutator protein MutT2 form 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-12-31 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.970, 60.710, 80.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.590 - 1.740 |
| R-factor | 0.19831 |
| Rwork | 0.196 |
| R-free | 0.23337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zrc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.313 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.590 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.145 | 0.441 |
| Number of reflections | 19279 | 2508 |
| <I/σ(I)> | 6.8 | 2.6 |
| Completeness [%] | 98.5 | 90.1 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 292 | 0.2 M Ammonium sulfate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% w/v PEG 8,000 |
