5ZRK
M. smegmatis antimutator protein MutT2 in complex with dCTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978560 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.780, 72.720, 58.320 |
| Unit cell angles | 90.00, 105.36, 90.00 |
Refinement procedure
| Resolution | 22.270 - 1.490 |
| R-factor | 0.13229 |
| Rwork | 0.130 |
| R-free | 0.17587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zrc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.454 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.270 | 1.570 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.057 | 0.561 |
| Number of reflections | 40931 | 5890 |
| <I/σ(I)> | 9.6 | 1.9 |
| Completeness [%] | 98.1 | 96.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 292 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v PEG 4000 |
