5ZRG
M. smegmatis antimutator protein MutT2 in complex with dCMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.080, 59.360, 31.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.080 - 1.300 |
| R-factor | 0.14451 |
| Rwork | 0.142 |
| R-free | 0.18365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zrc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.618 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.080 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.085 | 0.980 |
| Number of reflections | 30397 | 4297 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 97.9 | 96.2 |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 292 | 0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate Cryo: 5% ethylene glycol |
