5ZRE
Tyrosinase from Burkholderia thailandensis (BtTYR) at high pH condition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.781, 72.175, 117.409 |
| Unit cell angles | 90.00, 105.29, 90.00 |
Refinement procedure
| Resolution | 27.850 - 2.500 |
| R-factor | 0.2026 |
| Rwork | 0.199 |
| R-free | 0.26320 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.547 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.300 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 59146 | |
| <I/σ(I)> | 40.21 | |
| Completeness [%] | 97.0 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 18% (w/v) polyethyleneglycol 3350, 275 mM Ammonium iodide |
