5ZRC
Structural insights into the catalysis mechanism of M. smegmatis antimutator protein MutT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.8266 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.840, 59.870, 31.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.460 - 1.100 |
| R-factor | 0.14364 |
| Rwork | 0.142 |
| R-free | 0.17247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rrk |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.643 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.460 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.062 | 0.770 |
| Number of reflections | 49097 | 6891 |
| <I/σ(I)> | 16 | 3 |
| Completeness [%] | 95.9 | 93.4 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 292 | 0.1 M MES monohydrate pH 6.0, 20%(v/v) Jeffamine M-600 pH 7.0 |
