5ZR2
Crystal Structure of Phosphoserine Phosphatase Mutant (H9A) from Entamoeba histolytica in complex with Phosphoserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-10-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.107, 111.737, 178.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.280 - 2.950 |
| R-factor | 0.20929 |
| Rwork | 0.207 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zkk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.280 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 19911 | 930 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 97.9 | |
| Redundancy | 4.8 | |
| CC(1/2) | 0.910 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | PEG1500, MMT buffer pH 4.2, ammonium sulphate |
