5ZQL

crystal structure of human katanin AAA ATPase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	P 43
Unit cell lengths	97.810, 97.810, 72.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.481 - 3.007
R-factor	0.2752
Rwork	0.275
R-free	0.28520
Structure solution method	SAD
Starting model (for MR)	3b9p
RMSD bond length	0.005
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.075	0.389
Rmeas	0.034	0.294
Rpim	0.083	0.491
Number of reflections	13831	13831
<I/σ(I)>	10.8	4
Completeness [%]	94.5	85.4
Redundancy	4.6	2.1
CC(1/2)		0.055

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.3 M Lithium citrate acetate pH6.0 20% (v/v) PEG 3,350