5ZQ2

SidE apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2018-03-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	92.295, 129.364, 192.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.818 - 2.704
R-factor	0.2048
Rwork	0.203
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zq5
RMSD bond length	0.004
RMSD bond angle	0.771
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.166
Rmeas	0.173	0.980
Rpim	0.049	0.273
Number of reflections	63238
<I/σ(I)>	17.3
Completeness [%]	100.0
Redundancy	12.1
CC(1/2)	1.000	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Tacsimate