5ZQ1
Crystal structure of spRlmCD with U1939loop RNA at 3.10 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.386, 96.097, 114.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.100 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xj1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.390 | 3.100 |
| Rmerge | 0.163 | 0.086 | 0.844 |
| Rmeas | 0.173 | 0.092 | 0.899 |
| Rpim | 0.059 | 0.033 | 0.302 |
| Total number of observations | 84586 | ||
| Number of reflections | 10101 | 575 | 508 |
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 99.9 | 99 | 100 |
| Redundancy | 8.4 | 7.4 | 8.6 |
| CC(1/2) | 0.986 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES, pH 5.5, 0.15 M ammonium sulfate, 25% PEG4000 |
