5ZQ0

Crystal structure of spRlmCD with U747loop RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	47.412, 95.809, 114.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.1746
Rwork	0.173
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xj1
RMSD bond length	0.008
RMSD bond angle	1.359
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.080	0.040	0.477
Rmeas	0.083	0.042	0.499
Rpim	0.024	0.012	0.143
Number of reflections	35944	1986	1767
<I/σ(I)>	4.9
Completeness [%]	100.0	99.9	100
Redundancy	11.9	10.8	12.1
CC(1/2)		0.999	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES, pH 7.5, 36%(w/v) PEG600