5ZPS

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 10 at 288 K (1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	288
Detector technology	CCD
Collection date	2015-11-08
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.257, 64.797, 159.095
Unit cell angles	90.00, 117.09, 90.00

Refinement procedure

Resolution	40.540 - 1.748
R-factor	0.1527
Rwork	0.151
R-free	0.17660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.892
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.780
High resolution limit [Å]	1.750	4.750	1.750
Rmerge	0.073	0.025	0.690
Rmeas	0.085	0.030	0.807
Rpim	0.043	0.016	0.415
Total number of observations	660395
Number of reflections	172818	9049	8327
<I/σ(I)>	8.9
Completeness [%]	97.5	98.7	94.6
Redundancy	3.8	3.6	3.7
CC(1/2)		0.999	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES