5ZPR
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 9 at 288 K (2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 288 |
Detector technology | CCD |
Collection date | 2015-11-08 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.849, 64.320, 159.026 |
Unit cell angles | 90.00, 116.94, 90.00 |
Refinement procedure
Resolution | 40.429 - 1.922 |
R-factor | 0.1499 |
Rwork | 0.148 |
R-free | 0.18200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.870 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.950 |
High resolution limit [Å] | 1.920 | 5.210 | 1.920 |
Rmerge | 0.099 | 0.033 | 0.661 |
Rmeas | 0.117 | 0.039 | 0.803 |
Rpim | 0.062 | 0.021 | 0.450 |
Total number of observations | 444214 | ||
Number of reflections | 129153 | 6808 | 6320 |
<I/σ(I)> | 7.2 | ||
Completeness [%] | 97.5 | 99 | 95.9 |
Redundancy | 3.4 | 3.5 | 3.1 |
CC(1/2) | 0.998 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |