5ZPJ

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine at pH 6 at 288 K (1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	288
Detector technology	CCD
Collection date	2015-11-08
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	192.960, 64.597, 158.901
Unit cell angles	90.00, 116.89, 90.00

Refinement procedure

Resolution	35.430 - 1.647
R-factor	0.1554
Rwork	0.154
R-free	0.17530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.880
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.086	0.031	0.855
Rmeas	0.098	0.037
Rpim	0.052	0.019	0.525
Total number of observations	786257
Number of reflections	210429	10829	10375
<I/σ(I)>	7.8
Completeness [%]	99.9	99.6	99.7
Redundancy	3.7	3.5	3.6
CC(1/2)		0.999	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES