5ZPH

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH6 at 293K (2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	293
Detector technology	CCD
Collection date	2015-12-12
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.464, 64.505, 158.895
Unit cell angles	90.00, 117.06, 90.00

Refinement procedure

Resolution	40.437 - 1.720
R-factor	0.1545
Rwork	0.153
R-free	0.17910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.884
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.730	4.690	1.730
Rmerge	0.082	0.033	0.708
Rmeas	0.094	0.038	0.813
Rpim	0.046	0.019	0.398
Number of reflections	181520	9371	8811
<I/σ(I)>	7.6
Completeness [%]	98.9	99.4	98
Redundancy	4	3.8	4
CC(1/2)		0.999	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES