5ZPF
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 288 K (3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 288 |
Detector technology | CCD |
Collection date | 2015-12-12 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.379, 64.225, 158.773 |
Unit cell angles | 90.00, 117.08, 90.00 |
Refinement procedure
Resolution | 47.666 - 1.759 |
R-factor | 0.1563 |
Rwork | 0.155 |
R-free | 0.17890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.862 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.790 |
High resolution limit [Å] | 1.760 | 4.780 | 1.760 |
Rmerge | 0.102 | 0.044 | 0.714 |
Rmeas | 0.118 | 0.050 | 0.823 |
Rpim | 0.058 | 0.024 | 0.405 |
Total number of observations | 712645 | ||
Number of reflections | 172035 | 8881 | 8592 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 99.9 | 99.5 | 100 |
Redundancy | 4.1 | 3.9 | 4.1 |
CC(1/2) | 0.998 | 0.802 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |