5ZPD

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 288 K (1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	288
Detector technology	CCD
Collection date	2015-12-12
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.603, 64.612, 158.869
Unit cell angles	90.00, 117.09, 90.00

Refinement procedure

Resolution	39.666 - 1.665
R-factor	0.1539
Rwork	0.153
R-free	0.17180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.006
RMSD bond angle	0.895
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.700
High resolution limit [Å]	1.670	4.530	1.670
Rmerge	0.076	0.032	0.668
Rmeas	0.087	0.037	0.770
Rpim	0.042	0.018	0.379
Total number of observations	832090
Number of reflections	202832	10417	10064
<I/σ(I)>	8.7
Completeness [%]	99.9	99.6	99.7
Redundancy	4.1	3.9	4
CC(1/2)		0.999	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES