5ZPC
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 283 K (4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 283 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.789, 64.863, 158.885 |
| Unit cell angles | 90.00, 117.12, 90.00 |
Refinement procedure
| Resolution | 35.353 - 1.741 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.884 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.770 |
| High resolution limit [Å] | 1.740 | 4.720 | 1.740 |
| Rmerge | 0.102 | 0.040 | 0.768 |
| Rmeas | 0.119 | 0.048 | 0.884 |
| Rpim | 0.061 | 0.026 | 0.434 |
| Total number of observations | 655445 | ||
| Number of reflections | 174989 | 8980 | 8692 |
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 97.7 | 97 | 97.7 |
| Redundancy | 3.7 | 3.1 | 4 |
| CC(1/2) | 0.997 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
