5ZPC

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 283 K (4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	283
Detector technology	CCD
Collection date	2015-12-12
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.789, 64.863, 158.885
Unit cell angles	90.00, 117.12, 90.00

Refinement procedure

Resolution	35.353 - 1.741
R-factor	0.1987
Rwork	0.197
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.007
RMSD bond angle	0.884
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.770
High resolution limit [Å]	1.740	4.720	1.740
Rmerge	0.102	0.040	0.768
Rmeas	0.119	0.048	0.884
Rpim	0.061	0.026	0.434
Total number of observations	655445
Number of reflections	174989	8980	8692
<I/σ(I)>	8.1
Completeness [%]	97.7	97	97.7
Redundancy	3.7	3.1	4
CC(1/2)		0.997	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES