5ZP8
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 277 K (4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 283 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.590, 64.297, 158.791 |
| Unit cell angles | 90.00, 117.20, 90.00 |
Refinement procedure
| Resolution | 40.355 - 1.660 |
| R-factor | 0.1572 |
| Rwork | 0.156 |
| R-free | 0.17350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.878 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 4.500 | 1.660 |
| Rmerge | 0.088 | 0.041 | 0.851 |
| Rmeas | 0.104 | 0.049 | 0.993 |
| Rpim | 0.054 | 0.026 | 0.505 |
| Total number of observations | 747631 | ||
| Number of reflections | 201686 | 10390 | 10171 |
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 98.3 | 98 | 98.9 |
| Redundancy | 3.7 | 3.2 | 3.8 |
| CC(1/2) | 0.996 | 0.603 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
