5ZP4
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 10 at 288 K (2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 288 |
Detector technology | CCD |
Collection date | 2015-12-01 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.731, 64.838, 158.974 |
Unit cell angles | 90.00, 117.20, 90.00 |
Refinement procedure
Resolution | 35.135 - 1.799 |
R-factor | 0.1459 |
Rwork | 0.145 |
R-free | 0.16810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.859 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.799 | 4.880 | 1.800 |
Rmerge | 0.088 | 0.051 | 0.430 |
Rmeas | 0.102 | 0.060 | 0.504 |
Rpim | 0.053 | 0.032 | 0.260 |
Total number of observations | 609475 | ||
Number of reflections | 162845 | 8415 | 8077 |
<I/σ(I)> | 10.1 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 3.7 | 3.5 | 3.7 |
CC(1/2) | 0.989 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |