5ZP2
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 9 at 288 K (2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Collection date | 2015-12-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.765, 64.920, 158.953 |
| Unit cell angles | 90.00, 117.16, 90.00 |
Refinement procedure
| Resolution | 34.644 - 1.748 |
| R-factor | 0.1522 |
| Rwork | 0.151 |
| R-free | 0.17470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.861 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
| Rmerge | 0.114 | 0.057 | 0.823 |
| Rmeas | 0.131 | 0.066 | 0.950 |
| Rpim | 0.064 | 0.032 | 0.468 |
| Total number of observations | 731675 | ||
| Number of reflections | 177629 | 9167 | 8771 |
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 4.1 | 3.9 | 4 |
| CC(1/2) | 0.983 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
