5ZOW
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH 6 at 288 K (2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 288 |
Detector technology | CCD |
Collection date | 2015-12-01 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.581, 65.080, 158.828 |
Unit cell angles | 90.00, 117.06, 90.00 |
Refinement procedure
Resolution | 37.773 - 1.778 |
R-factor | 0.153 |
Rwork | 0.152 |
R-free | 0.17430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.885 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
Rmerge | 0.112 | 0.056 | 0.688 |
Rmeas | 0.128 | 0.064 | 0.794 |
Rpim | 0.062 | 0.031 | 0.392 |
Total number of observations | 699721 | ||
Number of reflections | 169015 | 8721 | 8492 |
<I/σ(I)> | 7.3 | ||
Completeness [%] | 100.0 | 99.8 | 99.9 |
Redundancy | 4.1 | 3.9 | 4.1 |
CC(1/2) | 0.976 | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |