5ZOV
Inward-facing conformation of L-ascorbate transporter UlaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 |
| Unit cell lengths | 107.336, 107.336, 113.288 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.986 - 3.333 |
| R-factor | 0.2753 |
| Rwork | 0.273 |
| R-free | 0.31650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rp9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.059 | 0.841 |
| Number of reflections | 20489 | 2129 |
| <I/σ(I)> | 23 | 1.87 |
| Completeness [%] | 97.6 | 100 |
| Redundancy | 4.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.28M CaCl2, 0.1M Tris-HCl, pH 7.5,42% PEG400 |
