5ZOU

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by ethylamine at pH6 at 288 K (1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	288
Detector technology	CCD
Collection date	2015-12-01
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	193.440, 64.244, 158.805
Unit cell angles	90.00, 117.12, 90.00

Refinement procedure

Resolution	40.353 - 1.680
R-factor	0.155
Rwork	0.154
R-free	0.17210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1iu7
RMSD bond length	0.006
RMSD bond angle	0.861
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.710
High resolution limit [Å]	1.680	4.560	1.680
Rmerge	0.085	0.050	0.636
Rmeas	0.097	0.058	0.734
Rpim	0.047	0.028	0.362
Number of reflections	195888	10176	9596
<I/σ(I)>	8.2
Completeness [%]	99.1	99.7	97.2
Redundancy	4.2	4	4
CC(1/2)		0.985	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES