5ZO8
Eg5 motor domain in complex with STLC-type inhibitor PVEI0021 (P21 type)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.293, 50.724, 83.355 |
| Unit cell angles | 90.00, 112.99, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.200 |
| R-factor | 0.20859 |
| Rwork | 0.205 |
| R-free | 0.24373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.420 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.290 |
| Number of reflections | 36080 | 4216 |
| <I/σ(I)> | 15.2 | 3.4 |
| Completeness [%] | 95.9 | 77.5 |
| Redundancy | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 24-34% PEG 3350, 0.1M MES, 0.2M Ammonium Sulfate |
