5ZO7
Kinesin spindle protein Eg5 in complex with STLC-type inhibitor PVEI0138
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-26 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.347, 50.580, 93.689 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 19.520 - 2.600 |
| R-factor | 0.23112 |
| Rwork | 0.225 |
| R-free | 0.28287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.543 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0048) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.083 | 0.377 |
| Number of reflections | 22457 | 3209 |
| <I/σ(I)> | 13.1 | 3.8 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 4.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% PEG 3350, 0.1M MES, 0.2M Ammonium Sulfate |
