5ZO5

active state of the nuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	I 4 2 2
Unit cell lengths	121.743, 121.743, 149.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.260 - 2.297
R-factor	0.189
Rwork	0.186
R-free	0.22450
RMSD bond length	0.002
RMSD bond angle	0.411
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2992: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.297	2.300
Number of reflections	24603
<I/σ(I)>	28.9
Completeness [%]	96.8
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris-HCl, pH 8.0, 1 M ammonium sulfate