5ZNM
Colicin D Central Domain and C-terminal tRNase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.352, 146.115, 45.763 |
| Unit cell angles | 90.00, 102.49, 90.00 |
Refinement procedure
| Resolution | 44.842 - 1.850 |
| R-factor | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.21230 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.973 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 63995 | |
| <I/σ(I)> | 14.1 | 1.8 |
| Completeness [%] | 96.4 | 93.9 |
| Redundancy | 5.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M HEPES sodium pH 7.5, 2.5% v/v Polyethylene glycol 400, 2.2 M ammonium sulfate |
