5ZNE

The crystal structure of immune receptor RGA5A_S of resistance protein Pia from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-09-10
Detector	MAC Science DIP-320
Wavelength(s)	0.9795
Spacegroup name	P 43 2 2
Unit cell lengths	55.619, 55.619, 78.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.350 - 1.780
R-factor	0.2189
Rwork	0.218
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dxs
RMSD bond length	0.006
RMSD bond angle	0.805
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.080	2.314
High resolution limit [Å]	1.780	2.234
Rmerge		0.012
Rmeas		0.017
Number of reflections	21743	6259
<I/σ(I)>	20.05
Completeness [%]	99.8	99.84
Redundancy	2	2
CC(1/2)		1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.18 M ammonium nitrate 20% PEG3350