5ZN5
X-ray structure of protein kinase ck2 alpha subunit H148A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.492, 45.547, 63.219 |
Unit cell angles | 90.00, 111.58, 90.00 |
Refinement procedure
Resolution | 34.920 - 1.700 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.22230 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.994 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.920 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.043 | 0.342 |
Number of reflections | 34398 | 3442 |
<I/σ(I)> | 16.5 | 2.3 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, 1.2M ammonium sulfate, 5% acetonitrile, 2mM DTT |