5ZN3
X-ray structure of protein kinase ck2 alpha subunit H148S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.590, 45.371, 63.415 |
| Unit cell angles | 90.00, 111.92, 90.00 |
Refinement procedure
| Resolution | 35.928 - 1.500 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.23020 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.054 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.380 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.036 | 0.540 |
| Number of reflections | 49865 | 7215 |
| <I/σ(I)> | 15.1 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, 1.2M ammonium sulfate, 5% acetonitrile, 2mM DTT |
