5ZMU
Crystal structure of a cis-epoxysuccinate hydrolase producing D(-)-tartaric acids
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 78.490, 94.250, 94.360 |
| Unit cell angles | 90.00, 95.90, 90.00 |
Refinement procedure
| Resolution | 40.350 - 1.500 |
| R-factor | 0.1491 |
| Rwork | 0.149 |
| R-free | 0.17020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y7d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | phenix.refine (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.350 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.115 | 0.664 |
| Number of reflections | 216906 | |
| <I/σ(I)> | 6.7 | 1.8 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M sodium chloride, 0.1 M HEPES pH7.0, 1.6 M ammonium sulfate |
