5ZMP
The structure of a lysine deacylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 |
| Unit cell lengths | 56.790, 56.790, 112.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.190 |
| Rwork | 0.173 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g11 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.700 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.214 | 1.130 |
| Rpim | 0.132 | 0.681 |
| Number of reflections | 18344 | 1342 |
| <I/σ(I)> | 8.7 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.26 M Sodium phosphate monobasic monohydrate, 0.14 M Potassium phosphate dibasic |
